Methods for calibrating chemical assays
US5801007A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1997 |
| Grant date | Sep 1, 1998 |
| Priority date | — |
| Expiry date | Apr 7, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for internally and externally photostandardizing chemical assays, e.g. an ATP-bioluminescence assay. A pre-determined amount of a photosensitive derivative of the analyte of interest is used in the assay protocol which releases a known amount of the free analyte when it is exposed to a flash of visible light of pre-determined duration and intensity. By monitoring the response of a test property of the assay to the release of a known quantity of analyte, a standard value can be calculated which allows either direct determination of the amount of analyte originally present in the assayed sample or production of a photocalibration series against which results of an assayed sample can be compared.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.