Hapten-labelled peptides

US5804371A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Eva Höss · Starnberg, DE
• Christoph Seidel · Weilheim, DE
• Ursula-Henrike Wienhues · Krailling, DE
• Elke Faatz · Huglfing, DE
• Urban Schmitt · Oberhausen, DE

Key dates

• Filing date: Jun 13, 1996
• Grant date: Sep 8, 1998
• Expiry date: Jun 13, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/82
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns a process for the production of hapten-labelled peptides which is characterized in that (a) a peptide with the desired amino acid sequence is synthesized on a solid phase from amino acid derivatives whose reactive side groups are blocked by protecting groups wherein the protecting groups on primary amino side groups are selected in such a way that, if desired, they can be selectively cleaved off, (b) protecting groups are cleaved to form at least one free primary amino group, (c) a hapten-active ester derivative is coupled to the at least one free primary amino group of the peptide and (d) if desired protecting groups that still remain are cleaved off, the hapten being selected from the group comprising sterols, bile acids, sexual hormones, corticoids, cardenolides, cardenolide-glycosides, bufadienolides, steroid-sapogenines and steroid alkaloids.