Nuclear factors associates with transcriptional regulation

US5804374A · kind A · utility

Assignees

• Massachusetts Insti. Technology · US
• Whitehead Insti. · US
• Pres. and Fellows of Harvard College · US

Inventors

• David Baltimore · Pasadena, US
• Ranjan Sen · Cambridge, US
• Phillip A. Sharp · Newton, US
• Harinder Singh · Chicago, US
• Louis M. Staudt · Silver Spring, US
• Jonathan LeBowitz · Zionsville, US
• Albert S. Baldwin · Chapel Hill, US
• Roger Clerc · Binningen, CH
• Lynn M. Corcoran · Melbourne, AU
• Patrick Baeuerle · Gauting, DE
• Michael J. Lenardo · Potomac, US
• Chen-Ming Fan · Boston, US
• Thomas P. Maniatis · Belmont, US

Key dates

• Filing date: Apr 6, 1995
• Grant date: Sep 8, 1998
• Expiry date: Apr 6, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/02
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Constitutive and tissue-specific protein factors which bind to transcriptional regulatory elements of Ig genes (promoter and enhancer) are described. The factors were identified and isolated by an improved assay for protein-DNA binding. Genes encoding factors which positively regulate transcription can be isolated and employed to enhance transription of Ig genes. In particular, NF-kB, the gene encoding NF-kB, IkB and the gene encoding IkB and uses therefor.