Stable aqueous reagent containing NAD
US5804403A · kind A · utility
2Cited by
8References
8Claims
0Family size
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Key dates
| Filing date | Jan 31, 1997 |
| Grant date | Sep 8, 1998 |
| Priority date | — |
| Expiry date | Jan 31, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A stable aqueous NAD reagent useful in the measurement of analytes in biological fluids, particularly ethanol and lactate. For the determination of alcohol, the reagent contains alcohol dehydrogenase, pyrazole, NAD, SUTTOCIDE A and an aliphatic, zwitterionic buffer having a secondary or tertiary amine group. Preferred buffers are TAPSO, AMPSO, POPSO, TAPS and DIPSO. For the determination of lactate, the reagent contains lactate dehydrogenase, pyrazole, NAD, SUTTOCIDE A and an aliphatic, zwitterionic buffer having a secondary or tertiary amine group.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.