Method of amplifying genes using artificial transposons in coryneform bacteria
US5804414A · kind A · utility
19Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 1, 1996 |
| Grant date | Sep 8, 1998 |
| Priority date | — |
| Expiry date | Jul 1, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.