High efficiency translation of mRNA molecules
US5807707A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 12, 1996 |
| Grant date | Sep 15, 1998 |
| Priority date | — |
| Expiry date | Feb 12, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An increased level of translation of a selected mRNA molecule is effected by coupling specific nucleotide sequences at the 5'- and 3' -ends of a nucleic acid molecule transcribable to or which itself is the mRNA molecule. The nucleotide sequence at the 5'-end is effective to increase the rate of translation initiation of the mRNA molecule in a cell while the nucleotide sequence at the 3'-end is effective to increase the period of translation of the mRNA molecule in a cell. The nucleotide sequence of the 3'-end is provided by a 3'-untranslated region (3'-UTR) of a gene, particularly that of .beta.-prolactin, or an effective fragment thereof. A polyadenylation sequence preferably is provided at the 3'-end of the 3'-UTR sequence. The 3'-UTR sequence provides mRNA stabilization independent of the poly A tail.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.