Methods and transformed mammalian lymphocyte cells for producing functional antigen-binding protein including chimeric immunoglobulin

US5807715A · kind A · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Sherie L. Morrison · Los Angeles, US
• Leonard A. Herzenberg · Stanford, US
• Vernon T. Oi · Beaumont, US

Key dates

• Filing date: Jun 27, 1994
• Grant date: Sep 15, 1998
• Expiry date: Jun 27, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods for producing functional immunoglobulin are provided. The methods involve transfecting and expressing exogenous DNA coding for the heavy and light chains of immunoglobulin. In some embodiments, chimeric immunoglobulins are provided having variable regions from one species and constant regions from another species by linking DNA sequences encoding for the variable regions of the light and heavy chains from one species to the constant regions of the light and heavy chains respectively from a different species. Introduction of the resulting genes into mammalian host cells under conditions for expression provides for production of chimeric immunoglobulins having the specificity of the variable region derived from a first species and the physiological functions of the constant region from a different species.