Method of producing human pluripotent hematopoietic colony stimulating factor

US5808008A · kind A · utility

Assignee

• Sloan Kettering Institute for Cancer Research · US

Inventors

• Karl Welte · New York, US
• Erich Platzer · Erlangen, DE
• Janice L. Gabrilove · New York, US
• Roland Mertelsmann · Chappaqua, US
• Malcolm A. S. Moore · New York, US

Key dates

• Filing date: Mar 12, 1997
• Grant date: Sep 15, 1998
• Expiry date: Mar 12, 2017

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Highly purified Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a glycoprotein (MW 19,600) constitutively produced by human tumor cells has been highly purified from low serum-containing conditioned medium to apparant homogeneity. Pluripotent CSF supports the growth of human mixed colonies (CFU-GEMM), granulocyte-macrophage colonies (CFU-GM), early erythroid colonies (BFU-E) and induces differentiation of human leukemic cells. The specific activity of the purified pluripotent CSF in the CFU-GM assay is 1.5.times.10.sup.8 U/mg protein.