Modified proenzymes as substrates for proteolytic enzymes
US5811252A · kind A · utility
40Cited by
5References
30Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 6, 1995 |
| Grant date | Sep 22, 1998 |
| Priority date | — |
| Expiry date | Jul 6, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Detection or determination of a protease in a sample by incubating the sample with a substrate of the protease and observing proteolytic cleavage of said substrate. The substrate is a modified proenzyme containing a recognition site, e.g., an activation site, cleavable by said protease. Proteolytic cleavage of the modified proenzyme is detected by observing the resulting activity using a suitable substrate of the activated proenzyme. The protease may be e.g. an aspartic protease or a metalloprotease, and the modified proenzyme e.g. pro-urokinase having a mutant activation site which is cleavable by the protease to be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.