Dry powder formulations of polynucleotide complexes

US5811406A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Francis C. Szoka, Jr. · San Francisco, US
• Alain Rolland · Palaiseau, FR
• Jinkang Wang · San Francisco, US

Key dates

• Filing date: Jun 9, 1995
• Grant date: Sep 22, 1998
• Expiry date: Jun 9, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/88
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Polynucleotide complexes are stabilized by adding a cryoprotectant compound and lyophilizing the resulting formulation. The lyophilized formulations are milled or sieved into a dry powder formulation which may be used to deliver the polynucleotide complex. Delivery of the polynucleotide to a desired cell tissue is accomplished by contacting the tissue with the powder to rehydrate it. In a preferred embodiment, a dry powder formulation is used to transfer genetic information to the cells of the respiratory tract.