Process for isolating and purifying nucleotide-activated sugars from biological sources

US5811539A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Andreas Seiffert-Stoeriko · Frankfurt, DE
• Brigitte Hoersch · Kriftel, DE
• Ruediger Marquardt · Frankfurt, DE
• Gerhard Kretzschmar · Eschborn, DE
• Johannes Meiwes · Idstein, DE

Key dates

• Filing date: Aug 30, 1995
• Grant date: Sep 22, 1998
• Expiry date: Aug 30, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleotides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromatographic method which uses silica gel as the stationary phase, and at a purity level which requires only a subsequent desalting step to obtain a substantially pure product.