Homogenous affinity assay for quantitative drug and metabolite determination
US5814449A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 28, 1996 |
| Grant date | Sep 29, 1998 |
| Priority date | — |
| Expiry date | May 28, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A homogenous affinity assay method, and sensor using such method, for the detection and measurement of analytes that uses an unmodified polyvalent receptor-bearing molecule and having a receptor with at least two binding sites that has an affinity for an analyte of interest, and two groups of molecules wherein one group of molecules is labeled with a molecule capable of generating a measurable response when in close proximity to a label molecule on the other group of molecules. In one embodiment, the groups of molecules also have grafted on them an analog to the analyte of interest. In the absence of the analyte of interest, members of the molecule groups are brought in close proximity to each other through affinity binding to receptor-bearing molecule. This affinity binding results in the formation of a binding complex consisting of members of the first and second groups of molecules and receptors and which results in a response between the labels on the molecules which is capable of measurement. In the presence of the analyte of interest, the binding complexes dissociate due to competitive replacement of the analog with the analyte of interest and affinity binding of the analyte …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.