Method for generating and screening useful peptides
US5814460A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 21, 1995 |
| Grant date | Sep 29, 1998 |
| Priority date | — |
| Expiry date | Feb 21, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6845
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention allows the generation and screening of a large population of peptides for the presence of peptides which bind a particular macromolecule or macromolecular complex with high affinity, and further allows the favored net synthesis of analyzable quantities of such peptides, by using is the "trap" a macromolecule or macromolecular complex for which binding of the peptide is desired. The starting mixture is preferably spiked with a peptide having some affinity for the target macromolecule so that mutation of the spike or "lead" peptide is favored. The development of improved binding peptides through scrambling may be dynamically monitored by initially binding the target with an insolubilized ligand, and then looking for an increase in the concentration of the target in the soluble phase as a result of the displacement of the reference ligand by scrambled peptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.