Method for the determination of anti-TSH receptor autoantibodies

US5814461A · kind A · utility

Assignee

• B.R.A.H.M.S. Diagnostica GmbH · DE

Inventors

• Andreas Bergmann · Berlin, DE
• Joachim Struck · Berlin, DE
• Shaul Kornfeld · Berlin, DE

Key dates

• Filing date: Jul 9, 1996
• Grant date: Sep 29, 1998
• Expiry date: Jul 9, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method for the determination of an analyte in a volume of a fluid sample, in particular of anti-TSH receptor autoantibodies in a patient serum, in which one or more determination reagent(s) which contains or which contain PA1 a) a predetermined amount of a binder (B) for the analyte (A) and PA1 b) a predetermined amount of a competitor (K) which is likewise bound by the binder (B), in such a way that the extent of its binding to the binder (B) is correlated with the simultaneous presence of the analyte (A) and/or its amount in the sample, the competitor (K) being labelled, or being capable of being labelled, with a label which permits its qualitative and/or quantitative determination, is or are added to the sample with the formation of a liquid reaction mixture, wherein PA1 c) the liquid reaction mixture is reacted simultaneously or subsequently with a solid phase to which a substance (Imm) for the selective immobilization of the amount of competitor (K) not bound to the binder (B) is bound, and optionally with a labelled reactant (Label) for the immobilized competitor (K), and, after a quantitative separation of the solid phase from the liquid reaction mixture, the amount of the c…