Tryptamine producing tryptophan decarboxylase gene of plant origin
US5814520A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 20, 1995 |
| Grant date | Sep 29, 1998 |
| Priority date | — |
| Expiry date | Apr 20, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/88
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In accordance with the present invention, there is provided an isolated and purified DNA fragment comprising a DNA sequence encoding a plant decarboxylase. Preferably, the DNA sequence encodes a plant tryptophan decarboxylase, more preferably from Catharanthus roseus. A 1747 bp cDNA clone was isolated by antibody screening of a cDNA expression library produced from poly A.sup.+ RNA found in developing seedlings of C. roseus. The invention also includes DNA sequences encoding a plant tryptophan decarboxylase which are synthetically produced to correspond substantially to the isolated and purified DNA sequence encoding the same enzyme. The nucleotide sequence of the synthetic DNA sequence is determined on the basis of codon degeneracy.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.