Cell lines from a transgenic mouse which express biologically active IX factor
US5814716A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 24, 1995 |
| Grant date | Sep 29, 1998 |
| Priority date | — |
| Expiry date | Feb 24, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/008
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cell lines have been prepared from transgenic mice comprised of a first exogenous DNA sequence encoding human factor IX which is controlled by a promoter region specific for hepatic cells and a second exogenous DNA sequence encoding an oncogene, such as the SV40 virus gene encoding the T-antigen or the mouse c-myc gene, which is controlled by a promoter region specific for hepatic cells. Promoter regions specific for hepatic cells included the promoter region for the factor IX gene and the promoter region for the .alpha.-1 antitrypsin gene. The cultured cells produce biologically active human factor IX which is then isolated from the culture media.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.