Heparan sulfate 2-0-sulfotransferase
US5817487A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 24, 1996 |
| Grant date | Oct 6, 1998 |
| Priority date | — |
| Expiry date | Jul 24, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/13
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Heparan sulfate 2-O-sulfotransferase having the following properties is obtained from, for example, CHO cells: PA1 (i) action: sulfate group is transferred from a sulfate group donor selectively to the hydroxyl group at C-2 position of iduronic acid residue of a sulfate group acceptor; PA1 (ii) substrate specificity: sulfate group is transferred to heparan sulfate or CDSNS-heparin, but sulfate group is not transferred to chondroitin, chondroitin sulfate, dermatan sulfate, and keratan sulfate; PA1 (iii) optimum reaction pH: about pH 5 to 6.5; PA1 (iv) optimum ionic strength: about 50 to 200 mM when sodium chloride is used; and PA1 (v) inhibition and activation: the activity of the enzyme is increased by protamine, the activity of the enzyme is inhibited by adenosine-3',5'-diphosphate, and the activity of the enzyme is scarcely affected by dithiothreitol at 10 mM or less.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.