Process for detecting evanescently excited luminescence

US5822472A · kind A · utility

Assignee

• Novartis AG · CH

Inventors

• Burkhard Danielzik · Ingelheim am Rhein, DE
• Gert Ludwig Duveneck · Bad Krozingen, DE
• Martin Heming · Saulheim, DE
• Dieter Neuschafer · Muttenz, CH
• Johannes Segner · Stromberg, DE

Key dates

• Filing date: Mar 4, 1997
• Grant date: Oct 13, 1998
• Expiry date: Mar 4, 2017

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/7743
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a process for determing lumnescence with a planar dielectric optical sensor platform which consists of a transparent substrate to which a thin transparent waveguiding layer is applied, which sensor platform is provided with a coupling grating for the input-coupling of the excitation light and the refractive index of the substrate is lower than the refractive index of the waveguiding layer, by briging a liquid sample as superstrate into contact with the layer, and measuring the luminescence produced by substances having luminescence properties in the sample, or by substances having luminescence properties immobilized on the layer, optoelectronically. The invention also relates to the use of the process in quantitative affinity sensing and to the use thereof for the quantitative determination of luminescent constituents in optically turbid solutions, and to sensor platform for carrying out the process.