Method for amplifying nucleic acid sequences by strand displacement using DNA/RNA chimeric primers

US5824517A · kind A · utility

Assignee

• BIOMERIEUX, INC. · FR

Inventors

• Philippe Cleuziat · L'Isle-d'Abeau, FR
• Bernard Mandrand · Villeurbanne, FR

Key dates

• Filing date: May 16, 1997
• Grant date: Oct 20, 1998
• Expiry date: May 16, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6844
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A target nucleic acid may be amplified in the presence of an enzymatic system including DNA polymerase, strand translocation and RNAse H activities by using a chimeric primer that includes, in the 5' to 3' direction, an RNA-type segment capable of hybridizing with a 3'-terminal segment of the target and a DNA-type segment capable of hybridizing with a segment adjacent to the 3'-terminal segment of the target and a DNA- or RNA-type primer capable of hybridizing with the 3'-terminal segment of the target. A cyclic amplification that may be implemented isothermally on the basis of either a DNA or an RNA target is achieved even when the terminals are not defined.