Shigella vector for delivering DNA to a mammalian cell

US5824538A · kind A · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Arthur Branstrom · Rockville, US
• Donata R. Sizemore · Brentwood, US
• Jerald C. Sadoff · Washington, US

Key dates

• Filing date: Sep 6, 1995
• Grant date: Oct 20, 1998
• Expiry date: Sep 6, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/822
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

We describe a bacterial delivery system for the delivery of DNA and antigens into cells. We constructed an attenuated bacterial vector which enters mammalian cells and ruptures delivering functional plasmid DNA, such as a mammalian expression plasmid, and antigens into the cell cytoplasm. This Shigella vector was designed to deliver DNA to colonic surfaces, thus opening the possibility of oral and other mucosal DNA immunization and gene therapy strategies. The attenuated Shigella is also useful as a vaccine for reducing disease symptoms caused by Shigella.