Method for detecting and quantitating nucleic acid impurities in biochemical preparations
US5824557A · kind A · utility
57Cited by
5References
13Claims
0Family size
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Key dates
| Filing date | Apr 2, 1996 |
| Grant date | Oct 20, 1998 |
| Priority date | — |
| Expiry date | Apr 2, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A homogeneous fluorescence-based nucleic acid detection and quantitation system is provided to measure nucleic acid in protein solutions. The process relies on the intercalation of a non-fluorescent dye into a double-stranded nucleic acid helix or single-stranded nucleic acid. The dye fluoresces after intercalation and the intensity is a direct measurement of the amount of nucleic acid present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.