Patent · US Expired

Synthesis of galactosylhydroxylysine

US5824786A · kind A · utility

0Cited by
0References
26Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 30, 1996
Grant dateOct 20, 1998
Priority date
Expiry dateAug 30, 2016

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/6887
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Galactosylhydroxylysine is a terminal degradation product of the bone matrix which thus serves as biochemical marker of bone resorption. A method of synthesizing galactosylhydroxylysine is provided. Antibodies against this synthetically prepared galactosylhydroxylysine conjugated to protein carriers were raised to establish methods to measure galactosylhydroxylysine in biological fluids. The antibodies of the invention recognize native galactosylhydroxylysine and are, for example, useful in the measurement of galactosylhydroxylysine as a biochemical marker of bone resorption in biological fluids. Testing of human serum or adolescent urine resulted in dose-dependent increases in signal intensity using the method of the invention.

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