Cross-linking oligonucleotides
US5824796A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 4, 1994 |
| Grant date | Oct 20, 1998 |
| Priority date | — |
| Expiry date | Nov 4, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/708
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
This invention is directed to novel substituted nucleotide bases with a crosslinking arm which accomplish crosslinking between specific sites on adjoining strands of oligonucleotides or oligodeoxynucleotides. The invention is also directed to oligonucleotides comprising at least one of these crosslinking agents and to the use of the resulting novel oligonucleotides for diagnostic and therapeutic purposes. The crosslinking agents of the invention are of the following formula (I'): EQU R.sub.1 --B--(CH.sub.2).sub.q --(Y).sub.r --(CH.sub.2).sub.m --A'(I') wherein, PA1 R.sub.1 is hydrogen, or a sugar moiety or analog thereof optionally substituted at its 3' or its 5' position with a phosphorus derivative attached to the sugar moiety by an oxygen and including groups Q.sub.1 Q.sub.2 and Q.sub.3 or with a reactive precursor thereof suitable for nucleotide bond formation; PA1 Q.sub.1 is hydroxy, phosphate or diphosphate; PA1 Q.sub.2 is .dbd.O or .dbd.S; PA1 Q.sub.3 is CH.sub.2 --R', S--R', O--R', or N--R'R"; PA1 each of R' and R" is independently hydrogen or C.sub.1-6 alkyl; PA1 B is a nucleic acid base or analog thereof that is a component of an oligonucleotide; PA1 Y is a functional lin…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.