Nucleic acids encoding BSSL variants

US5827683A · kind A · utility

Assignee

• ASTRA CO., LTD. · SE

Inventors

• Lars Gustav Blackberg · Wasserburg am Inn, DE
• Michael Edlund · Wasserburg am Inn, DE
• Stig Lennart Hansson · Wasserburg am Inn, DE
• Olle Hernell · Umeå, SE
• Lennart Gustav Lundberg · Lindome, SE
• Mats Stromqvist · Wasserburg am Inn, DE
• Jan Törnell · Mölndal, SE

Key dates

• Filing date: Mar 1, 1994
• Grant date: Oct 27, 1998
• Expiry date: Mar 1, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention discloses nucleic acids encoding variant Bile Salt Stimulated Lipase (BSSL; EC 3.1.1.1). The encoded variant BSSL enzymes maintain catalytic activity but contain fewer glycosylation sites that full-length BSSL. This reduced glycosylation facilitates purification and characterization of recombinant BSSL proteins.