Cryopreservation of human adult and fetal pancreatic cells and human platelets
US5827741A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 1996 |
| Grant date | Oct 27, 1998 |
| Priority date | — |
| Expiry date | Nov 19, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K35/39
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The combination of trehalose and dimethyl sulfoxide is an unusually effective cryoprotectant for islets and islet-like cell clusters, as well as platelets. Islets and ICCs, when cooled through the thermotropic phase transition in the presence of this combination of treatment agents and then returned to physiologic temperature, retain their functionality. Likewise, platelets can be similarly cooled and warmed without undergoing premature activation. In general, trehalose can be incorporated into eukaryotic cells in general by suspending the cells in a trehalose solution and either cooling or warming the solution through the thermotropic transition of the cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.