Walk-through mutagenesis
US5830650A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 30, 1995 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | May 30, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/034
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a protein to produce library of mutants. The method is based on the premise that certain amino acids play crucial role in the structure and fuction of proteins. Libraries can be generated which contain a high proportion of the desired mutants and are of reasonable size for screening. This libraries can be used to study the role of specific amino acids in protein structure and function and to develop new or improved proteins and polypeptides such as enzymes, antibodies, single chain antibodies and catalytic antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.