Oligonucleotide sizing using cleavable primers
US5830655A · kind A · utility
276Cited by
9References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 26, 1996 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Apr 26, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a 3' portion of the primer (linked to an extension product) can be released from an upstream 5' portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.