Patent · US Expired

In situ recombinant PCR within single cells

US5830663A · kind A · utility

193Cited by
0References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 13, 1994
Grant dateNov 3, 1998
Priority date
Expiry dateJul 13, 2014

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/10
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed is a method of treating a heterogeneous population of cells to link together copies of two or more nucleic acid sequences from at least some of the cells, the arrangement being such that copies of the DNA sequences from an individual cell are preferentially linked in the vicinity of the nucleic acid from which the copies are derived. Also disclosed are recombinant proteins expressed by the method of the invention and kits for performing said method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.