Method for the detection of target nucleic acid

US5830664A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Viola Rosemeyer · Wavre, BE
• Rudolf Seibl · Penzberg, DE

Key dates

• Filing date: Jul 17, 1995
• Grant date: Nov 3, 1998
• Expiry date: Jul 17, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6865
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for the sensitive detection of a target nucleic acid by hybridization with a probe nucleic acid. The latter contains a part which can hybridize with the target nucleic acid and a nucleic acid-specific part which does not hybridize with the target nucleic acid. The method further comprises cleavage of the probe nucleic acid, hybridization of a cleavage product of the probe nucleic acid containing the part that does not hybridize with the target nucleic acid with a matrix nucleic acid containing a part that can be hybridized with the cleavage product and a part that cannot be hybridized with the probe nucleic acid. The method also comprises the determination of the hybrid consisting of the cleavage product and the matrix nucleic acid and a reagent kit suitable for this purpose.