Unitary sandwhich enzyme immunoassay cassette device and method of use
US5830680A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 1996 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Aug 15, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A novel enzyme sandwich immunoassay cassette comprising a microporous membrane support having coated thereon: PA1 (a) a conductive metal layer; and PA1 (b) a capture antibody layer over the conductive metal layer. A device using this cassette to perform an enzyme sandwich immunoassay for a predetermined analyte comprises: PA1 (a) a first chamber into which an antibody for the analyte is placed, wherein the antibody is conjugated with an enzyme and wherein the enzyme is capable of catalyzing the production of an electrochemically detectable species; PA1 (b) a second chamber adjoining the first chamber and into which a substrate for the enzyme is placed, wherein the substrate is capable of producing, when catalyzed by the enzyme, an electrochemically detectable species; and PA1 (c) separating the first and second chambers, a microporous membrane support as described above, wherein the conductive metal layer of the microporous membrane acts as an electrode to detect, directly or indirectly, the presence of the electrochemically detectable species. Finally, a method is provided for performing an enzyme sandwich immunoassay using the above-described device and cassette.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.