Increased amounts of substances by modifying a microorganism to increase production of NADPH from NADH
US5830716A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 29, 1996 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Apr 29, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of producing a target substance by microbial fermentation, where the natural ability of the microorganism to produce NADPH from NADH is increased. The present method is useful for producing a wide variety of materials, such as amino acids, antibiotics, vitamins, growth factors and other physiologically active substances. The microorganism is modified to increase production of NADPH from NADH in order to increase the amount of an active substance in the culture medium. The modification of the microorganism results in the increased production of the amount of nicotinamide nucleotide transhydrogenase expressed by the microorganism. Further, the increase in the production of NADPH from NADH is increased by increasing the number of copies of a gene encoding a nicotinamide nucleotide transhydrogenase enzyme. The copies of the gene are so produced to a number effective to increase the amount of the enzyme expressed by the microorganism, thereby increasing the enzyme activity of the microorganism. Therefore, the amount of an active substance produced by the modified microorganism in the culture medium is increased and thus, isolated from the culture medium as a physiologically act…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.