Printing oligonucleotide arrays using deprotection agents solely in the vapor phase

US5831070A · kind A · utility

Assignee

• AFFYMETRIX, INC. · US

Inventors

• R. Fabian W. Pease · Stanford, US
• Glenn McGall · Mountain View, US
• Martin J. Goldberg · Saratoga, US
• Richard P. Rava · San Jose, US
• Stephen P. A. Fodor · Palo Alto, US
• Virginia Goss · Lompoc, US
• Lubert Stryer · Stanford, US
• James L. Winkler · Sunnyvale, US

Key dates

• Filing date: Apr 19, 1996
• Grant date: Nov 3, 1998
• Expiry date: Apr 19, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B60/14
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.