Combined use of two expression cassettes for the production of a protein of interest
US5834237A · kind A · utility
18Cited by
0References
34Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 7, 1996 |
| Grant date | Nov 10, 1998 |
| Priority date | — |
| Expiry date | Oct 7, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a system for producing a protein of interest in a host cell comprising the combined use of two expression cassettes, one of which is for expressing a useful DNA fragment controlled by a thiamine-regulable promoter region, while the other is for expression an activator gene. The present invention also concerns expression cassettes comprising a useful DNA fragment controlled by a promoter region derived from the Schizosaccharomyces pombe pho-4 gene, as well as the vectors and cells comprising such an expression cassette. A novel method for the production of a useful protein is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.