Quantification of nucleic acid
US5834255A · kind A · utility
80Cited by
2References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 7, 1995 |
| Grant date | Nov 10, 1998 |
| Priority date | — |
| Expiry date | Feb 7, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of quantifying a target nucleic acid in a test sample by adding to the test sample a known number of molecules of a corresponding nucleic acid comprising a well-defined mutant sequence. Said mutant sequence being discriminatory from the target nucleic acid. Subsequently a competitive amplification reaction of the nucleic acid is performed after which quantification of the amplified nucleic acid is performed by a differential detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.