Heparan sulfate 6-O-sulfotransferase
US5834282A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 21, 1995 |
| Grant date | Nov 10, 1998 |
| Priority date | — |
| Expiry date | Jul 21, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/13
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a heparan sulfate 6-O-sulfotransferase for selectively introducing sulfate group into hydroxyl group at C-6 of glucosamine of heparin and heparan sulfate, having the following properties: (i) sulfate group is selectively transferred from a sulfate group donor to hydroxyl group at C-6 of N-sulfoglucosamine residue; (ii) sulfate group is transferred to CDSNS-heparan, but sulfate group is not transferred to chondroitin and chondroitin-4-sulfate; (iii) optimum reaction pH is in a range of pH 6-7; (iv) optimum ionic strength is in a range of 0.1-0.3 M (in the case of sodium chloride); and (v) the enzyme activity is inhibited by dithiothreitol and adenosine-3',5'-diphosphate, and activated by protamine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.