Methods of identifying biologically active receptor-binding peptides

US5837460A · kind A · utility

Assignees

• The Trustees of the University of Pennsylvania · US
• The Wistar Institute · US

Inventors

• Joan M. Von Feldt · Wilmington, US
• Thomas Kieber-Emmons · Little Rock, US
• David B. Weiner · Yeadon, US
• William V. Williams · Havertown, US

Key dates

• Filing date: Sep 3, 1996
• Grant date: Nov 17, 1998
• Expiry date: Sep 3, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/965
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of identifying peptides which mimic biologically active proteins is disclosed. The method comprises the steps of making a recombinant antibody library from genetic material obtained from an animal which has been immunized against antibodies that bind to the biological active protein to the mimicked. Recombinant antibodies are screened to identify antibodies which compete with the biological active protein. Peptides which comprise the recombinant antibody's CDR sequences are synthesized. Synthetic peptides which mimic GM-CSF are also disclosed.