PCR-based cDNA substractive cloning method
US5837468A · kind A · utility
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4References
4Claims
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Key dates
| Filing date | May 19, 1997 |
| Grant date | Nov 17, 1998 |
| Priority date | — |
| Expiry date | May 19, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described in which PCR-based cDNA libraries with anchored ends are made, a PCR-based cDNA subtracted (PCSUB) library is produced therefrom, and the ends of a cDNA clone are isolated, via PCR, from an anchored-ends library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.