DNA encoding and biosynthetic process for the preparation of chemical compounds, lantibiotics

US5837485A · kind A · utility

Assignee

• Dr. Karl Thomae GmbH · DE

Inventors

• Karl-Dieter Entian · Oberursel, DE
• Friedrich Gotz · Tübingen, DE
• Norbert Schnell · Pasadena, US
• Johannes Augustin · Tübingen, DE
• Germar Engelke · Frankfurt am Main, DE
• Ralf Rosenstein · Reutlingen, DE
• Cortina Kaletta · Hersbruck, DE
• Cora Klein · Offenbach, DE
• Bernd Wieland · Rottenburg am Neckar, DE
• Thomas Kupke · Tübingen, DE
• Gunther Jung · Tübingen, DE
• Roland Kellner · Heppenheim, DE

Key dates

• Filing date: Feb 22, 1995
• Grant date: Nov 17, 1998
• Expiry date: Feb 22, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/825
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A bacterial host is described which is transformed by a plasmid coding for a polypeptide precursor wherein the host comprises a multi-enzyme complex capable of reacting with the expressed polypeptide precursor to produce a polypeptide comprising at least one dehydroamino acid and/or at least one lanthionine bridge. A process for producing a polypeptide comprising at least one dehydroamino acid and/or at least one lanthionine bridge, such as gallidermin, is also described. A plasmid capable of transforming a bacterial host is additionally described. Also disclosed are recombinant DNA molecules which specify Epi B, Epi C, Epi D, Epi P and Epi Q, enzymes which are involved in the biosynthesis of lantibiotic epidermin.