Vector containing viral gene transcribed by RNA polymerase III and methods for use
US5837503A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1996 |
| Grant date | Nov 17, 1998 |
| Priority date | — |
| Expiry date | Jun 12, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13043
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant vector containing a cassette for transcription by RNA polymerase III is disclosed. The cassette contains a viral gene transcribed by RNA polymerase III into which an oligonucleotide, a DNA fragment, has been inserted between or outside the boxes A and B constituting the promoter of the viral gene is provided. A method for the intracellular production of an RNA fragment in vitro or in vivo, in which eukaryotic cells containing RNA polymerase III are transfected or infected with a vector according to the invention, containing as oligonucleotides a DNA fragment corresponding to the reverse transcript of the RNA, and in which the eukaryotic cells thus transfected or infected are cultured in a suitable culture medium is also provided. The use of the vectors as a medicinal product is further provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.