Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
US5837509A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 1, 1995 |
| Grant date | Nov 17, 1998 |
| Priority date | — |
| Expiry date | Mar 1, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless structural gene as a promoter probe gene into a population of a lactic acid bacterium, methods of constructing a recombinant lactic acid bacterium comprising a regulatable promoter by using the above method, a recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promoter not natively associated with the gene, the use of such a recombinant lactic acid bacterium and recombinant plasmids comprising a regulatable lactic acid bacterial promoter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.