Method of purification of viral vectors
US5837520A · kind A · utility
88Cited by
1References
27Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 7, 1995 |
| Grant date | Nov 17, 1998 |
| Priority date | — |
| Expiry date | Mar 7, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a method for purifying viral vectors containing therapeutic genes for use in gene therapy. The invention comprises a method of purification from a cell lysate of a recombinant viral vector containing a therapeutic gene, which comprises: a) treating said lysate with an enzymatic agent that selectively degrades both unencapsulated DNA and RNA; b) chromatographing the treated lysate from step a) on a first resin; and c) chromatographing the eluant from step b) on a second resin; wherein one resin is an anion exchange resin and the other is an immobilized metal ion chromatography (IMAC) resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.