Method for lysing cells
US5837529A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 1996 |
| Grant date | Nov 17, 1998 |
| Priority date | — |
| Expiry date | Apr 15, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method for lysing cells. The method comprises simultaneously flowing a cell suspension and a lysis solution through a static mixer, wherein the cells exit the static mixer lysed. In another aspect of the present invention, the invention relates to a method for precipitating cell components, protein, and nucleic acids from a cell lysate or other solution containing precipitable material. The method comprises simultaneously flowing a cell lysate or other protein containing solution and a precipitating solution through a static mixer, wherein the lysate or protein solution exits the static mixer with its precipitable components precipitated. In another aspect of the present invention, the invention relates to a method where the two above-mentioned methods above are combined by using static mixers in series.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.