Method for inhibiting thrombosis in a patient whose blood is subjected to extracorporeal circulation

US5839443A · kind A · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Eric Rose · Dobbs Ferry, US
• David M. Stern · Great Neck, US
• Ann Marie Schmidt · Franklin Lakes, US
• Talia Spanier · New York, US

Key dates

• Filing date: May 16, 1996
• Grant date: Nov 24, 1998
• Expiry date: May 16, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/9645
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides an assay to monitor antithrombic activity of a Factor IXa compound infused into circulation of a patient which includes: (a) obtaining Factor IXa-deficient plasma; (b) mixing the plasma from step (a) with diatomaceous and with plasma from the patient; (c) incubating the mixture with a source of lipid and calcium chloride under conditions suitable for clot formation; and (d) measuring time necessary for clot formation in the incubate, thereby monitoring the antithrombic activity of the Factor IXa compound infused into the circulation of the patient.