Apparatus and process for electroelution of a gel containing charged macromolecules
US5840169A · kind A · utility
Inventor
Key dates
| Filing date | Mar 13, 1995 |
| Grant date | Nov 24, 1998 |
| Priority date | — |
| Expiry date | Mar 13, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44739
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for electroelution of a gel containing charged macromolecules, such as proteins or polynucleotides, comprising the steps of providing a plurality of adjacent parallel chambers having a trapezoidal cross-section, placing a gel containing the charged macromolecules onto first open sides of the chambers, placing a semipermeable membrane onto second open sides of the chambers, filling the chambers with an elution buffer and applying a voltage difference across the chambers so that charged macromolecules in the gel migrate into the elution buffers in the chambers. Also, an apparatus for electroelution of a gel containing charged macromolecules having, preferably, a plurality of adjacent parallel chambers having a trapezoidal cross-section and vents for removing the product without disassembling the apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.