Polyvinyl alcohol (PVA) based covalently bonded stable hydrophilic coating for capillary electrophoresis
US5840388A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 1997 |
| Grant date | Nov 24, 1998 |
| Priority date | — |
| Expiry date | May 22, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/31935
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A coated microcapillary column for high performance electrophoresis is disclosed. A preferred microcapillary includes a fused silica capillary column, the inner surface of the column having an interconnected polymeric coating of a polyvinyl alcohol (PVA) based polymer covalently attached to the column wall by Si--O--Si bonds. The resulting coated microcapillary column has good hydrolytic and pH stability and minimizes electroosmotic flow and interactions between sample components and the capillary wall. Also disclosed are a method of forming a polymeric coating layer for any surface by polymerizing the appropriate organic compounds in an organic solvent and a method of forming a column with a hydrophilic polymeric coating by directly converting an attached hydrophobic polymeric coating material to a hydrophilic polymeric coating material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.