Patent · US Expired

Method of detecting amplified nucleic sequences in cells by flow cytometry

US5840478A · kind A · utility

3Cited by
5References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 18, 1994
Grant dateNov 24, 1998
Priority date
Expiry dateMay 18, 2014

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N15/14
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The field of this invention is the detection and isolation of specific amplified DNA sequences by flow cytometry. More particularly, this invention relates to the detection of these specific amplified DNA sequences in cells so as to allow quantitation of viral burden of patients infected with a virus. The method is particularly adapted to detection of HIV-1 proviral DNA sequences and the assessment of activity of the virus in a cell.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.