DNA fragment, vector containing the DNA fragment, transformant transformed with the vector and process for producing protein using the vector
US5840518A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 29, 1994 |
| Grant date | Nov 24, 1998 |
| Priority date | — |
| Expiry date | Apr 29, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/036
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA fragments having a nucleotide sequence represented by x and vectors containing the DNA fragment, wherein the nucleotide sequence x encodes an amino acid sequence represented by any one of the following formulae 1 to 8: PA0 formula 1 AVLPQEEEGSG, PA0 formula 2 AVLPQEEEGSGGGQLVTEVTKKEDSG, PA0 formula 3 AVLDQEEEGSG, PA0 formula 4 AVLPQEEEGDG, PA0 formula 5 AVLPQEEEGSGD, PA0 formula 6 AVLPQEEEGSGDD, PA0 formula 7 AVLPQEEEGSGDDD, and PA0 formula 8 ADDPQEEEGSG. Expression of a protein of interest and its secretion from host cells into the extracellular milieu can be increased by expressing or secreting the protein of interest in the form of a fusion protein with a specified polypeptide encoded by the DNA fragment of the present invention using the vector of the present invention, by means of recombinant DNA techniques in which the inventive DNA fragment is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.