Method for producing seeds and plants thereof involving an in vitro step
US5840557A · kind A · utility
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19Claims
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Key dates
| Filing date | Mar 27, 1997 |
| Grant date | Nov 24, 1998 |
| Priority date | — |
| Expiry date | Mar 27, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing transgenic seeds and plants, which comprises isolating, from anthers, microspores in their uninucleate stage and removing tissue in which they are embedded, culturing the isolated microspores in a nutrient solution, transferring foreign genetic material into the isolated microspores to obtain transformed microspores, bringing about complete maturation of the transformed microspores to obtain transformed pollen grains in vitro, pollinating receiver plants with the transformed pollen grains and obtaining seeds from the pollinated receiver plants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.