Method of simultaneously detecting amplified nucleic acid sequences and cellular antigens in cells
US5843640A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 30, 1995 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | Aug 30, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/14
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In one aspect, the present invention provides an in situ process of simultaneously detecting a specific predetermined nucleic acid sequence and a specific predetermined cellular antigen in the same cell. In accordance with that process, the antigen is labeled with a biotin- or DNP-tagged antibody that specifically immunoreacts with the antigen, the specific nucleic acid sequences in the cell are amplified, the amplified nucleic acid sequences are labeled with a fluorescently-tagged nucleic acid probe that specifically hybridizes to the amplified nucleic acid sequences, and the labeled nucleic acid sequences and labeled cellular antigen are detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.