Nucleic acid detection and amplification by chemical linkage of oligonucleotides
US5843650A · kind A · utility
Inventor
Key dates
| Filing date | May 1, 1995 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | May 1, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention and kits are directed to a method of amplifying and detecting single or double-stranded target nucleic acid molecules in a test sample. Amplification is accomplished through the use of a minimum of two oligonucleotide probe complement pairs, wherein members oligonucleotide probes from both pair of oligonucleotide probe complement pairs form a minimum of two oligonucleotide probe pairs, at least one of which is complementary to a given portion of a target nucleic acid sequence which act as template. One of the oligonucleotide probes of each oligonucleotide probe pair have an additional protecting sequence which is not complementary to the target sequence. These additional protecting sequences are preferably complementary to each other. Chemical functionality groups attached to the oligonucleotide probes covalently combine the probes to form a joined oligonucleotide product. The joined oligonucleotide product is formed without the use of enzymes. The reactivity of the chemical functionality groups on each probe is target dependent. The chemical functionality group on each probe is prevented from reacting with other chemical functionality groups on other probes u…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.